Calorimeter-Fermentor Combination for Investigations on Microbial Steady State Cultures
نویسندگان
چکیده
Flow Microcalorimetry, E. coli K12, Aerobic Chemostat Culture The combination of a 3-liter fermentor with a flow-through calorimeter is described. Minimum time-lags are less than 10 s corresponding to maximum thermal peak disturbances of ~ 4 jiW. The set up shows an excellent long time stability. Aerobic growth of Escherichia coli K12 in batch cultures was used to test the equipment. Precision is ± 2.1% with regard to the total heat release of 4.94 kJ • I-1 and ± 2.5% with respect to the maximum heat flow of 609 mW • l-1. The accuracy is comparable with calorimeters using commercial flow mixing cells.
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